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This software provides a hybrid GO-based semantic similarity algorithm (HRSS, Hybrid Relative Specificity Similarity) for evaluating the functional similarity between GO terms or gene products. The software uses the pre-downloaded GO database files and the GO annotation files. It allows the users to set organisms, and evidence codes ignored. The software is composed of five modules, getGODAG, getGOAnno, hrssmatrix, hrsstps and hrsspps.